Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline powder, pH 7.4, for preparing 1 L solutions | MFCD00131855 | 1PAK
Phosphate buffered saline powder, pH 7.4, for preparing 1 L solutions | MFCD00131855 | 1PAK
HORIZON DISCOVERY BIOSCIENCES LIMITED 10MM TRIS-HCL BUFFER PH 7.4
NC2372517 10MM TRIS-HCL BUFFER PH 7.4
Boston Bioproducts Inc MES-SDS Buffer (20X) - 1L
The 20X MES-SDS Running Buffer is formulated specifically for running proteins on Bis-Tris gels for separating small to medium-sized proteins under denaturing conditions in SDS-PAGE. When reconstituted from the 20X concentrate, this buffer maintains a stable pH of approximately 7.3. The MES-SDS Running Buffer enables faster and efficient resolution of well-defined protein bands in comparison to other running buffers in SDS-PAGE experiments.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline BioPerformance Certified, pH 7.4 | MFCD00131855 | 10PAK
Phosphate buffered saline BioPerformance Certified, pH 7.4 | MFCD00131855 | 10PAK
Revvity Health Sciences Inc DELFIA L*R wash concentrate (25x) Standard, 250 mL
DELFIA L*R wash concentrate (25x) Standard, 250 mL
Teknova 1X PBS with 200mM Sodium Chlor
1X PBS with 200mM Sodium Chloride, 0.001% P-188, 1000mL, Sterile 8 pack.
Research Products International Corp PBS 200ml Tablets 500 Tab
PBS, 200ml Tablets refer to pre-weighed tablets used to prepare a specific volume of PBS (Phosphate Buffered Saline) solution.
The buffer tablets are commonly used buffer solution in biological and biochemical research. PBS serves as a versatile buffer for various applications, including cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. It helps maintain a stable pH and osmolarity, providing optimal conditions for biological reactions and preserving the integrity of cells and biomolecules.
The PBS, 200ml Tablets are designed to simplify the preparation process of PBS solutions. Each tablet contains pre-determined amounts of sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic. By using these tablets, researchers can save time and ensure accuracy by eliminating the need to weigh and measure individual components.
![eMolecules 23141-25-5 | STRICTOSAMIDE | AstaTech | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502460896.JPG-250.jpg)
eMolecules 23141-25-5 | STRICTOSAMIDE | AstaTech | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441
STRICTOSAMIDE | AstaTech | 23141-25-5 | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441